IT ALL STARTED WHEN…

This is Jill Malone, Robert’s wife, writing this history. We have been married for 46 years ,and I was intricately involved in science at that time. Furthermore, we have kept all the records of these events, which include lab books, patent disclosures, patent applications, correspondence, drafts of papers, data, and more from that period. This history is based on. my own recollections as well as Robert’s, and it is verified by the written documentation, which can be accessedks below.

Dr. Malone is the inventor of mRNA vaccines (and DNA vaccines). He also discovered lipid-mediated and naked RNA transfection technologies. This is important because his role in inventing these technologies gave him the initial credibility to speak on the subject of mRNA vaccines. When mainstream media and the government decided to censor him and deny his role in these discoveries, they had a purpose. That purpose was to prevent him from being viewed as a legitimate expert. Those newspaper articles are still front and center on Google. The deep state agents within our government can not handle the truth and must censor and delegitimize dissenting voices.

It all began when he was at the Salk Institute during 1987 and 1988. There, he pioneered in vitro RNA transfection and in vivo RNA transfection in frog embryos and mice.

This resulted in his seminal paper: Cationic liposome-mediated RNA transfection RW Malone, PL Felgner, IM Verma. Proceedings of the National Academy of Sciences (PNAS) 86 (16), 6077-6081

His filed patent and disclosures from the Salk included in-vivo RNA transfection, mRNA as a drug, mRNA production, transient gene therapy, and methods for mRNA stabilization. These are available for review and can be found in the link below this statement.

When he left the Salk Institute, he moved his research to Vical in 1988. He brought over mRNA constructs and reagents developed at the Salk Institute by Dr. Malone. The first in-vivo mammalian mRNA experiments were designed by Dr. Malone in January 1989, which included dosing amounts for these experiments. Since he had brought his constructs, plasmids, and reagents from the Salk Institute, he was able to quickly ramp up his research program at Vical, where he was the only employee actively working on this technology at the time. The first in-vivo mouse experiments were performed in February 1989 when mRNA and DNA were sent to Dr. Jon Wolff via FedEx. Dr.Wolff and his technicians at the University of Wisconsin injected mice and rats.

The initial patent disclosures for RNA and DNA vaccination were authored by Dr. Malone in 1988 and 1989. Dr. Malone was also an inventor of DNA vaccines during this period. He wrote the patent disclosures (documents linked below). Dr. Rhodes and Dr. Malone designed the first mRNA vaccine experiments, which were conducted by Dr. Gary Rhodes in consultation with Dr. Malone.

THE INITIAL mRNA VACCINE EXPERIMENT EVER PERFORMED WAS FIRST CARRIED OUT IN 1989 AND IS WRITTEN UP IN THE ORIGINAL PATENTS. THERE ARE IMAGES OF THESE EXPERIMENTS FROM THE PATENTS FOUND AT THE BOTTOM OF THIS PAGE.

This body of work resulted in nine patents and numerous publications, yielding about 14,,000 citations for this work.

The paper that showed that first in-vivo RNA transfection data:

• Cationic liposome-mediated RNA transfection, R W Malone, P L Felgner, and I M Verma PNAS August 1, 1989 86 (16) 6077-6081;

The paper was the first to show data for DNA and RNA transfection side by side for in-vivo (the first paper for in-vivo DNA):

Direct gene transfer into mouse muscle in vivo. Wolff JA, Malone RW, et al. Science. 1990;247(4949 Pt 1):1465-8. Cited in 4,750 articles, is the result of that work.

In 1989, research was conducted that led to the groundbreaking patents on mRNA vaccination, all with a priority date of March 21, 1989. This is the same priority date as the Salk Patent application, indicating that the two institutions collaborated. These patents represent the first published research on mRNA vaccination. The titles and links to the patents are provided in the documents below. These patents include proof-of-principle experiments on mRNA vaccines that clearly demonstrate the invention's effectiveness and represent the first experiments to show this.
Induction of a protective immune response in a mammal by injecting a DNA (or RNA) sequence, Patent US5589466A, Priority date 3/21/1989

Vical informed Robert that they would license the Salk Technology (refer to the Vical meeting minutes in the linked document). However, they hired Robert’s thesis advisor from Salk, and shortly after, Salk abandoned the patent, leading Vical never to pursue a license from them. Due to an employee contract with Vical, Robert was prevented from working commercially in the field for a decade. Vical asserted that all Salk research was conducted at Vical and subsequently issued a cease and desist letter.

Dr. Malone continued his research on mRNA and DNA vaccination during the 1990s, culminating in a mucosal patent issued in 2000 with a priority date of 1996. He also helped revolutionize the field of cationic liposomes for use in RNA and DNA vaccinations. This work was so far ahead of its time that only now is the world turning to mucosal mRNA vaccination as a method of immunization. Unfortunately, the toxicity issues from the nano lipid particles noted by Dr. Malone in his 1990s research were never resolved. Furthermore, the addition of pseudouridine to the mRNA, which creates synthetic mRNA, has increased toxicity and adverse events. These issues were not resolved prior to or during the clinical trials. The government regulators have made little effort to investigate these problems.

For a list of some of his works, please refer to the publications linked at the end of this page.

Scientifically trained at UC Davis, UC San Diego, and the Salk Institute's Molecular Biology and Virology laboratories, Dr. Malone received his. medical training at Northwestern University Medical School (Clinical Research Post Graduate) and in Pathology at UC Davis. He has 100 peer-reviewed publications and has been an invited speaker at approximately 50 conferences.

For the full backstory on the discovery of mRNA vaccination, as written by Dr. Malone’s partner and wife, please download the PDF located in the section below: